منابع مشابه
Molecular cloning of eukaryotic glycoprotein and glycolipid glycosyltransferases: a survey.
The rapidity with which molecular sequence data are gathered continues to grow. The result is that, for many workers, it is increasingly difficult to keep abreast of the current state of play of molecular cloning, even for those genes that encode proteins of special interest. The clear success of the various worldwide genome projects has made this even more apparent, and by the end of 1996 the ...
متن کاملCloning, expression, and spectral analysis of mouse betatrophin
Background: Betatrophin, a novel secretory protein from liver and fatty tissues, is believed to be involved in lipid and glucose metabolism. However, its precise physiological role remains unclear. Here, we report the cloning, expression, and purification steps of mouse betatrophin in a prokaryotic system, followed by its structural analysis. Methods: Specific cloning primers were...
متن کاملCloning and expression of the enzymatic region of Streptococcal hyaluronidase
Objective(s): Streptococcus pyogenes produces extracellular hyaluronidase enzyme. This enzyme is directly associated with the spread of the organism during infection. The objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. Materials and Methods: The enzymatic region of hyaluronidase gene was detecte...
متن کاملCloning and Expression of Protease 2A from Coxsakievirus B3
Protease 2A (2Apro) of coxsackievirus B3 (CVB3) plays a major role in viral replication. In case of infection, viral proteins are being synthesized from viral mRNA using host biosynthesis machinery. 2Apro of virus, after being synthesized, exhibits two critical functions, cleavage of viral proteins and breaking eukaryotic initiation factor 4G. The enzyme plays an essential role in viral replic...
متن کاملCloning, Expression, Purification and CD Analysis of Recombinant Human Betatrophin
Betatrophin is a member of the angiopoietin-like (ANGPTL) family that has been implicated in both triglyceride and glucose metabolism. The physiological functions and molecular targets of this protein remain largely unknown; hence, a purified available protein would aid study of the exact role of betatrophin in lipid or glucose metabolism. In this study, we cloned the full-length cDNA of betatr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Glycobiology
سال: 1996
ISSN: 0959-6658,1460-2423
DOI: 10.1093/glycob/6.7.683